RESUMO
INTRODUCTION: This study aimed to detect anti-Leptospira spp antibodies and Leptospira DNA in domestic dogs. METHODS: Blood and urine from 106 dogs were evaluated by microscopic agglutination test (MAT) and polymerase chain reaction (PCR), respectively. RESULTS: Six (5.7%) and one (1%) animals were positive by MAT and PCR, respectively. CONCLUSIONS: These results show a low prevalence of infection by Leptospira spp. The absence of positive results for the Icterohaemorrhagiae serogroup indicates the small relevance of these dogs as sources of human leptospirosis.
Assuntos
Testes de Aglutinação/veterinária , Doenças do Cão/diagnóstico , Leptospira/classificação , Leptospirose/veterinária , Reação em Cadeia da Polimerase/veterinária , Animais , Brasil/epidemiologia , Doenças do Cão/epidemiologia , Doenças do Cão/microbiologia , Cães , Leptospira/genética , Leptospira/imunologia , Leptospirose/diagnóstico , Leptospirose/epidemiologia , Leptospirose/microbiologia , PrevalênciaRESUMO
Abstract INTRODUCTION: This study aimed to detect anti-Leptospira spp antibodies and Leptospira DNA in domestic dogs. METHODS: Blood and urine from 106 dogs were evaluated by microscopic agglutination test (MAT) and polymerase chain reaction (PCR), respectively. RESULTS: Six (5.7%) and one (1%) animals were positive by MAT and PCR, respectively. CONCLUSIONS: These results show a low prevalence of infection by Leptospira spp. The absence of positive results for the Icterohaemorrhagiae serogroup indicates the small relevance of these dogs as sources of human leptospirosis.
Assuntos
Animais , Cães , Testes de Aglutinação/veterinária , Reação em Cadeia da Polimerase/veterinária , Doenças do Cão/diagnóstico , Leptospira/classificação , Leptospirose/veterinária , Brasil/epidemiologia , Prevalência , Doenças do Cão/microbiologia , Doenças do Cão/epidemiologia , Leptospira/genética , Leptospira/imunologia , Leptospirose/diagnóstico , Leptospirose/microbiologia , Leptospirose/epidemiologiaRESUMO
Toxoplasmosis and leishmaniasis are two worldwide zoonoses caused by the protozoan parasites Toxoplasma gondii and Leishmania spp., respectively. This report describes the clinical and laboratorial findings of a co-infection with both parasites in a 4-year-old female dog suspected of ehrlichiosis that presented anemia, thrombocytopenia, hypoalbuminemia, hyperglobulinemia, tachyzoite-like structures to the lung imprints, and polymerase chain reaction (PCR) results positive for T. gondii (kidney, lung, and liver) and Leishmania spp. Co-infection with Toxoplasma gondii and Leishmania braziliensis was confirmed by sequencing; restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) confirmed an atypical T. gondii genotype circulating in dogs that has been reported to cause human congenital toxoplasmosis.
Assuntos
Doenças do Cão/parasitologia , Leishmania braziliensis/genética , Leishmaniose/veterinária , Toxoplasma/genética , Toxoplasmose Animal/parasitologia , Animais , Coinfecção/parasitologia , Coinfecção/veterinária , DNA de Protozoário , Doenças do Cão/diagnóstico , Cães , Feminino , Genótipo , Leishmaniose/diagnóstico , Leishmaniose/parasitologia , Reação em Cadeia da Polimerase/veterinária , Polimorfismo de Fragmento de Restrição , Toxoplasmose Animal/diagnósticoRESUMO
Toxoplasmosis and leishmaniasis are two worldwide zoonoses caused by the protozoan parasites Toxoplasma gondii and Leishmania spp., respectively. This report describes the clinical and laboratorial findings of a co-infection with both parasites in a 4-year-old female dog suspected of ehrlichiosis that presented anemia, thrombocytopenia, hypoalbuminemia, hyperglobulinemia, tachyzoite-like structures to the lung imprints, and polymerase chain reaction (PCR) results positive for T. gondii (kidney, lung, and liver) and Leishmania spp. Co-infection with Toxoplasma gondii and Leishmania braziliensis was confirmed by sequencing; restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) confirmed an atypical T. gondii genotype circulating in dogs that has been reported to cause human congenital toxoplasmosis.
Assuntos
Animais , Cães , Feminino , Doenças do Cão/parasitologia , Leishmania braziliensis/genética , Leishmaniose/veterinária , Toxoplasma/genética , Toxoplasmose Animal/parasitologia , Coinfecção/parasitologia , Coinfecção/veterinária , DNA de Protozoário , Doenças do Cão/diagnóstico , Genótipo , Leishmaniose/diagnóstico , Leishmaniose/parasitologia , Polimorfismo de Fragmento de Restrição , Reação em Cadeia da Polimerase/veterinária , Toxoplasmose Animal/diagnósticoRESUMO
This study reports an unusual case of deforming mandibular osteomyelitis in a cow caused by Trueperella (Arcanobacterium) pyogenes, on the face of the ventrolateral caudal portion of the right branch of the mandible. Fragment aspired of lesion by fine needle allowed cytological characterization, isolation and identification of T. pyogenes. Radiographic examination showed marked periosteal reaction in the right mandible, numerous lytic areas and cortical bone destruction. Despite of treatment based on in vitro antimicrobial sensitivity test, it was recommended the euthanasia due to progressive worsening of the cow's condition. Multiple abscesses were observed in the mandibular region at necropsy. Pyogranuloma was characterized in histological exam. Sampled material collected from the lesion after necropsy resulted in microbiological reisolation of T. pyogenes .
Relata-se caso incomum de osteomielite mandibular deformante em vaca, causada por Trueperella (Arcanobacterium) pyogenes, na face ventro-lateral da porção caudal do ramo direito da mandíbula. A punção aspirativa de fragmento da lesão permitiu a caracterização citológica, o isolamento microbiano e identificação de T. pyogenes. Exame radiográfico mostrou acentuada reação periodontal na mandíbula direita, com predomínio de áreas líticas e destruição da cortical óssea. Apesar da instituição do tratamento baseado no teste de sensibilidade microbiana in vitro, foi recomendada a eutanásia, em virtude da piora progressiva do estado geral do animal. No exame post-mortem foram observados múltiplos abscessos na lesão que, histologicamente, foi caracterizada como piogranuloma. A colheita de material da região mandibular afetada, após a necropsia, resultou no reisolamento microbiológico de T. pyogenes .
